Overview

Peptides are typically supplied as a dry powder (lyophilized). To use them, they must be reconstituted (mixed with a liquid solvent).

Step-by-Step Process

1. 
   Wash your hands and work in a clean environment.
   
2. 
   Use sterile bacteriostatic water (or the solvent specified for that peptide).
   
3. 
   Remove the protective cap from the peptide vial and disinfect the rubber stopper with an alcohol swab.
   
4. 
   Using a sterile syringe, draw up the desired amount of solvent.
   
5. 
   Insert the needle into the peptide vial at a slight angle, pointing towards the inside wall of the vial.
   
6. 
   Slowly release the solvent so it runs gently down the glass wall, avoid squirting directly onto the powder.
   
7. 
   Do not shake the vial. Gently roll or swirl it until the powder is fully dissolved.
   
8. 
   Label the vial with the date of reconstitution and store it as recommended (commonly refrigerated at 2–8°C).

Step-by-Step Process

1. 
   Wash your hands thoroughly and work in a clean, controlled environment.
   
2. 
   Select the correct needle and syringe size for the planned research use.
   
3. 
   Swab the top of the peptide vial with alcohol to sterilize it.
   
4. 
   Draw air into the syringe equal to the amount of solution you intend to withdraw.
   
5. 
   Insert the needle into the vial and push the air in, this equalises pressure.
   
6. 
   Invert the vial and slowly withdraw the required amount of solution into the syringe.
   
7. 
   Remove air bubbles by gently tapping the syringe and pushing the air out.
   
8. 
   Prepare the injection site according to the research protocol: Clean the skin with an alcohol swab and allow it to dry.
   
9. 
   Administer the solution via subcutaneous or intramuscular injection, depending on the research requirements specified in the protocol.
   
10. 
    Dispose of needles and syringes immediately in an approved sharps container.

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